Render Target: STATIC
Render Timestamp: 2024-11-07T10:11:11.429Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:58:54.000
Product last modified at: 2024-10-17T12:15:31.302Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZAP (E5V4D) Rabbit mAb #33750

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 80, 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZAP (E5V4D) Rabbit mAb recognizes endogenous levels of total ZAP protein. This antibody recognizes ZAP isoforms 1 and 2.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp210 of human ZAP protein.

    Background

    ZAP (Zinc finger CCCH-type antiviral protein 1, ZC3HAV1, PARP13) is an RNA-binding protein which inhibits the replication of certain viruses, including HIV-1, by preventing the accumulation of viral RNA in the cytoplasm (1-5). ZAP binds to specific viral mRNAs through the CCCH zinc finger motifs located on its N terminus, and recruits exosomes and decapping enzymes to degrade the target RNA (3-5). ZAP also interferes with the interaction between eIF4A and eIF4G on target mRNA, causing translational repression (6). Isoform 2, the shorter isoform also known as ZAPS, acts as a stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I as part of the innate antiviral immune response (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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