Render Target: STATIC
Render Timestamp: 2024-10-11T09:53:31.107Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:38.242
Product last modified at: 2024-10-03T12:45:08.140Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

ZO-1 (6B6E4) Rat mAb #15652

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H Dg
    SENSITIVITY Endogenous
    MW (kDa) 220
    Source/Isotype Rat IgG2a
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Dg-Dog 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZO-1 (6B6E4) Rat mAb recognizes endogenous levels of total ZO-1 protein.

    Species Reactivity:

    Human, Dog

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to sequences within the PDZ1 and PDZ2 domains of human ZO-1.

    Background

    Tight junctions, or zona occludens (ZO), form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins, such as occludin and claudin, to the actin cytoskeleton (reviewed in 2). ZO-1 and ZO-2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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