Render Target: STATIC
Render Timestamp: 2025-03-19T11:12:12.395Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:07:44.108
Product last modified at: 2025-02-03T20:45:13.059Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

ZPR1 (LG1) Mouse mAb #2014

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 51
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZPR1 (LG1) Mouse mAb detects endogenous levels of total ZPR1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with purified recombinant human ZPR1.

    Background

    The zinc finger protein ZPR1 (ZNF259) binds to epidermal growth factor receptor (EGFR) and is localized to both cytoplasm and nucleus. The zinc fingers found in ZPR1 and the tyrosine kinase domain of EGFR mediate the interaction between ZPR1 and the receptor (1). ZPR1 translocates from the cytoplasm to nucleus following mitogen (i.e. EGF) stimulation (2,3). ZPR1 also interacts with translation elongation factor eEF1A in vivo following EGF treatment (3). The interaction between the zinc finger protein and elongation factor is important for cell proliferation. Cells lacking ZPR1 exhibit abnormal nucleolar function, suggesting that ZPR1 is required for cell viability and nucleolar function in dividing cells (3). ZPR1 knockout mice exhibit significant neurodegeneration, and reduced or altered expression of ZPR1 may contribute to spinal muscular atrophy, a disorder characterized by degeneration of spinal cord neurons (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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