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32948
PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit
Proteomic Analysis Products
PTMScan

PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit #32948

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PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit: Image 1

This chart shows the underlying motif ditribution in a PhosphoScan® LC-MS/MS experiment using 1526 nonredundant Lys-C digested peptides generated from mouse embryo and immunoprecipitated using PTMScan® Basophilic Kinase Motif [(R/X)(R/X)Xp(S/T)] Immunoaffinity Beads. Phospho-threonine peptides comprised 17% of peptides in this data set while phospho-serine contributed 83%.

PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit: Image 2

The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 1526 nonredundant Lys-C digested peptides derived from mouse embryo immunoprecipitated with PTMScan® Basophilic Kinase Motif [(R/X)(R/X)Xp(S/T)] Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position surrounding the central phosphorylated residue.

Product Includes Volume (with Count)
PTMScan(R) Basophilic Kinase Motif [(R/X)(R/X)Xp(S/T)] IAP Beads 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 10 x 600 µl
PTMScan® Limited Use License 1 x 1 ml

Storage

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® Proteomics Services, please visit https://www.cellsignal.com/services/index.html.

Background

As an integral part of the machinery of cellular function, proteins undergo regulation by a variety of post-translational modifications. One of the most prevalent and widely studied PTMs is serine/threonine phosphorylation. Prominent kinases targeting consensus substrate motifs account for tens of thousands of known and predicted sites on more than 13,000 human proteins (1-3). Cell Signaling Technology has developed phospho-Ser/Thr motif antibodies for proteomic profiling of kinase substrates. Arg-directed or AGC-family kinases including PKA, PKG, PKC, Akt, p70 S6 kinase, AMPK, and RSK and are characterized by preference for basic amino acids (Lys or Arg) especially Arg at position -3 relative to the phosphorylated Ser or Thr (1,4). Akt, p70S6 kinase and RSK additionally share specificity for Lys or Arg at position -5 (5). CST™ has developed a Basophilic PTMScan® kit comprising a pool of antibodies that recognize basophilic kinase substrate motifs as a powerful proteomics analysis tool for investigating the regulation of phosphorylation by Arg-directed kinases, as well as for high throughput kinase drug discovery.

  1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  2. Rao, R.S. and Møller, I.M. (2012) Biochim Biophys Acta 1824, 405-12.
  3. Goel, R. et al. (2012) Mol Biosyst 8, 453-63.
  4. Montminy, M. (1997) Annu. Rev. Biochem. 66, 807-822.
  5. Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.