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  3. PTMScan® Carbamidomethyl Cysteine Motif (CAM-C) Kit

PTMScan® Carbamidomethyl Cysteine Motif (CAM-C) Kit #23682

Additional Information

This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.

Contact the CST Proteomics Group

    Product Information

    Storage

    Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Product Description

    PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation, ubiquitination, acetylation, and methylation, among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® products and services, please visit Proteomics Resource Center.

    Background

    Cysteine alkylation is often performed with iodoacetamide (IAA) or chloroacetamide (CAA) to generate a carbamidomethyl (CAM) modification. Because alkylation occurs on reduced cysteines, disulfide-bonded cysteines must first be treated with dithiothreitol (DTT) or another reducing agent. The alkylation reaction is irreversible. Consequently, alkylated cysteines are unable to reassociate with each other. If the sulfhydryl group of a cysteine is already modified by another compound, that cysteine will not be able to react with IAA to form the carbamidomethyl modification. The alkylation reaction is rapid, although its efficiency can be influenced by the type of denaturant used (1).

    Proteins are often derivatized to prevent disulfide bond scrambling, which can lead to artifactual bands in gel electrophoresis, and to minimize protease inaccessibility of cysteine-containing regions, which can limit coverage in mass spectrometry-based proteomics. In light of various studies describing potential non-cysteine reactivities of IAA and CAA and re-evaluating the effectiveness of such reactions, users should consider the benefits of cysteine derivatizations in relation to their specific experimental goals (2-4).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
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    PTMScan is a registered trademark of Cell Signaling Technology, Inc.
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