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17011
PTMScan® Carboxymethyl/Carboxyethyl Lysine Motif Kit
Proteomic Analysis Products
PTMScan

PTMScan® Carboxymethyl/Carboxyethyl Lysine Motif Kit #17011

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PTMScan® Carboxymethyl/Carboxyethyl Lysine Motif Kit: Image 1
Motif analysis of tryptic peptides from 293 human embryonic kidney cells enriched and identified by PTMScan® Carboxymethyl/Carboxyethyl Lysine Motif Kit. Untreated protein was pooled with glyoxal and methylglyoxal treated protein in a 4:1 mass ratio prior to trypsin digestion, where five milligrams of the resulting peptides were immunoprecipitated with PTMScan® Carboxymethyl/Carboxyethyl Lysine Immunoaffinity Beads. Orbitrap Q Exactive mass spectrometer analysis identified a total of 9,432 non-redundant modified sites.
Product Includes Volume (with Count)
PTMScan® Carboxymethyl/Carboxyethyl Lysine Immunoaffinity Beads 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 10 x 600 µl
PTMScan® Limited Use License 1 x 1 ea

Storage

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1).

Background

Carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) are two protein post-translational modifications that arise from non-enzymatic pathways and represent examples of advanced glycation end products (AGEs) (1). The association between CML/CEL levels with glucose and oxidant stress levels underlies the use of these modifications as biomarkers for various disease conditions, including diabetes and osteoporosis (2). The Maillard reaction between monosaccharides and lysines can form a Schiff base intermediate that rearranges to become CML/CEL. In addition, fragmentation of the Schiff base intermediate and oxidation of amino acids and polyunsaturated fatty acids produce reactive species, such as glyoxal and methylglyoxal, which react with lysine to form CML/CEL. While CML/CEL exists as free and protein-incorporated forms, in vitro treatment with protein-incorporated CML/CEL in particular induces diverse effects, including reduced serotonin release in gastric cells, impaired calcium homeostasis in cardiac cells, and increased reactive oxygen intermediates in endothelial cells (3-5). These processes depend on receptor for AGE (RAGE) signaling.
  1. Nevin, C. et al. (2018) Sci Rep 8, 9002.
  2. Asadipooya, K. and Uy, E.M. (2019) J Endocr Soc 3, 1799-1818.
  3. Holik, A.K. et al. (2018) Food Funct 9, 3906-3915.
  4. Petrova, R. et al. (2002) J Mol Cell Cardiol 34, 1425-31.
  5. Yan, S.D. et al. (1994) J Biol Chem 269, 9889-97.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]
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