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65666
PTMScan® Cell Growth and Death Antibody Sampler Kit
Proteomic Analysis Products

PTMScan® Cell Growth and Death Antibody Sampler Kit #65666

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This PhosphoSitePlus® (PSP) logo was generated from a PhosphoScan® LC-MS/MS experiment using 1111 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 and pervanadate and immunoprecipitated using Immunoaffinity beads from PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit #12267. The PSP logo reflects the relative prevalence of an amino acid in each position relative to the phospho-serine background in the human proteome. Residues represented above the x-axis are enriched relative to their expected frequency in this background. For more information on motif analysis using PSP, please visit www.phosphosite.org.

The motif logo was generated from a PTMScan® LC-MS/MS experiment using 1044 nonredundant tryptic peptides with carboxy-terminal aspartates derived from human HeLa cells treated with Staurosporine #9953 (1 μM, 3 hr) to induce apoptosis. Peptides were immunoprecipitated with Immunoaffinity beads from PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit #12810. The logo represents the relative frequency of amino acids in each position leading up to the carboxy-terminal aspartate.

The motif logo was generated from a PhosphoScan® LC-MS/MS experiment using 754 nonredundant tryptic peptides derived from mitotic HeLa cells that were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using Immunoaffinity beads from PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit #14990. The motif logo demonstrates the relative abundance of amino acids in a given position within this data set.

The motif logo shows the amino acid distributions around the sites recognized using Immunoaffinity beads from PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit #4652.

The motif logo shows the amino acid distribution around the sites recognized using Immunoaffinity beads from PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit #5563.

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. An alternate PTMScan® IAP Buffer Plus Detergent #9992 which may reduce nonspecific interactions is available separately. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.

Storage:

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com.

Background

For more information on our PTMScan® Motif Antibody Kits, please visit www.cellsignal.com. Specific product background information can be found on the web page for each PTMScan® Motif Antibody kit component. Cleaved Caspase Substrate Motif background is described on the webpage for PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit #12810. Phospho-MAPK/CDK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit #4652. Phospho-MAPK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit #14990. Phospho-Akt Substrate Motif background is described on the webpage for PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit #5563. Phospho-ATM/ATR Substrate Motif background is described on the webpage for PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit #12267.

  1. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
For Research Use Only. Not For Use In Diagnostic Procedures.

AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
PhosphoSitePlus is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.