News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

PTMScan® Motif Antibody Kits
New Format-Same Price

Now 10 Assays Per Kit

Order Now

Pricing & Additional Information

To learn more about our Proteomics Kits and Services, including pricing, please answer a few questions for our proteomics group.

Contact the CST Proteomics Group  

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Product Includes Cap Color Volume (with Count)
PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Immunoaffinity Beads Blue 2 x 80 µl
PTMScan® Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Blue 2 x 80 µl
PTMScan®Phospho-MAPK Substrate Motif [PXpTP] Immunoaffinity Beads Blue 2 x 80 µl
PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Blue 2 x 80 µl
PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Immunoaffinity Beads Blue 2 x 80 µl
PTMScan® IAP Buffer (10X) 9993 White 10 x 600 µl
PTMScan® Limited Use License 1 x  license

Product Usage Information

Cells are lysed in a urea-containing buffer,

cellular proteins are digested by proteases, and the resulting

peptides are purified by reversed-phase, solid-phase extraction.

Peptides are then subjected to immunoaffinity purification

using a PTMScan® antibody conjugated to protein A agarose

beads. Unbound peptides are removed through washing, and

the captured PTM-containing peptides are eluted with dilute acid.

Reversed-phase purification is performed on microtips to desalt

and separate peptides from antibody prior to concentrating the

enriched peptides for LC-MS/MS analysis. CST recommends

the use of PTMScan® IAP Buffer #9993 included in the kit. An

alternate PTMScan® IAP Buffer Plus Detergent #9992 which

may reduce nonspecific interactions is available separately. A

detailed protocol and Limited Use License allowing the use of the

patented PTMScan® method is included with the kit.


Storage: Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com.


For more information on our PTMScan® Motif Antibody Kits, please visit www.cellsignal.com. Specific product background information can be found on the web page for each PTMScan® Motif Antibody kit component. Cleaved Caspase Substrate Motif background is described on the webpage for PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit #12810. Phospho-MAPK/CDK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit #4652. Phospho-MAPK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit #14990. Phospho-Akt Substrate Motif background is described on the webpage for PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit #5563. Phospho-ATM/ATR Substrate Motif background is described on the webpage for PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit #12267.


1.  Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.



For Research Use Only. Not For Use In Diagnostic Procedures.
AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
PhosphoSitePlus is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

65666
PTMScan® Cell Growth and Death Antibody Sampler Kit