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PDP - Template Name: PTMScan
PDP - Template ID: *******5df2d2a

PTMScan® Multi-Pathway Enrichment Kit #75676

Pricing & Additional Information

To learn more about our Proteomics Kits and Services, including pricing, please answer a few questions for our proteomics group.

Contact the CST Proteomics Group

    Product Information

    Product Usage Information

    Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Monoclonal Antibody mix conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit.

    Storage

    Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Product Description

    PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content/content.jsp?id=ptmscan-services.

    Background

    PTMScan® Multi-Pathway Enrichment Kit introduces Cell Signaling Technology's PTMScan® Direct Multi-Pathway proteomics service to the end user in a convenient and easy-to-use kit format. Based on the patented PTMScan® method, the kit includes an array of site-specific antibodies conjugated to protein A beads for peptide immunoaffinity purification. The kit is designed for the screening, discovery, and quantitation of thousands of proteins and phosphorylation sites on hundreds of critical signaling proteins spanning multiple signaling pathways. These include cell cycle and checkpoint control, PI3 Kinase and Akt signaling, MAPK and JNK cascades, T-cell and B-cell receptor signaling, as well as some housekeeping targets. The kit can also be used as the enrichment reagent for customized targeted assays by including heavy isotope-labeled synthetic peptides to a subset of validated targets.

      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      AcetylScan is a registered trademark of Cell Signaling Technology, Inc.
      MethylScan is a registered trademark of Cell Signaling Technology, Inc.
      PhosphoScan is a registered trademark of Cell Signaling Technology, Inc.
      UbiScan is a registered trademark of Cell Signaling Technology, Inc.
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.