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91336
PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Kit

PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Kit #91336

Chart Image 1

This chart shows the distribution of underlying phospho-peptide motifs in a PhosphoScan® LC-MS/MS experiment using 261 tryptic peptides generated from mouse embryo and immunoprecipitated using PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Immunoaffinity Beads.

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Motif Logo Image 2

The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 261 nonredundant tryptic peptides derived from mouse embryo and immunoprecipitated using PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Immunoaffinity Beads.

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Product Includes Cap Color Volume (with Count)
PTMScan® IAP Buffer (10X) 9993 White 10 x 600 µl
PTMScan(R) Phospho-Thr-X-Arg Motif [pT(P/X)R] IAP Beads Blue 10 x 80 µl
PTMScan® Limited Use License 1 x  license

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method are included with the kit.

Storage:

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content.jsp?id=ptmscan-services.

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine residues that are followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K (4,5). Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by an arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing a serine or threonine with Arg or Lys at the -3, -2 and +2 positions (6,7). Cell Signaling Technology has developed antibodies that bind to phosphorylation sites containing the T(P/X)R motif for discovery and proteome-wide profiling of kinase substrates.

  1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  4. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
  6. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
  7. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
For Research Use Only. Not For Use In Diagnostic Procedures.

AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.

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