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60055
Urea, Ultrapure, PTMScan® Qualified
Proteomic Analysis Products
Buffer

Urea, Ultrapure, PTMScan® Qualified #60055

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Urea, Ultrapure, PTMScan® Qualified: Image 1

Product Usage Information

For best results Urea Lysis Buffer should be prepared fresh prior to each experiment when working with PTMScan® protocols.

To make Urea Lysis buffer:

1. Add 45 ml of reverse osmosis deionized (RODI) or equivalent water and 10 ml of 200 mM HEPES buffer (#44686) and mix well until the urea goes completely into solution.

2. Add 1 ml of 100X stock of fresh Phosphatase Inhibitor Cocktail (#5870) just before use. Measure the volume and bring up to the final volume of 100 ml with RODI water.

Note: Dissolving urea is an endothermic reaction that decreases the temperature of the solution. This reaction will cause the urea to precipitate. Allow the solution to warm to room temperature to ensure all of the urea dissolves. The cell lysis step should take place at room temperature to prevent the urea from precipitating out of solution.

See PTMScan® protocols for additional details.

Solutions and Reagents

1X Urea lysis buffer is 9M Urea at 100 ml final volume.

Storage

Store at room temperature. The final buffer solution can be stored for up to 2 months at -80°C.

Product Description

Urea is an organic compound that is highly soluble in water with strong protein denaturing properties. These properties make it ideal for use in preparation of Urea Lysis buffer for preparing cell and tissue extracts prior to PTMScan®-based applications. Urea, Ultrapure, PTMScan® Qualified from Cell Signaling Technology is offered in a convenient 54.05g size that allows for the preparation of 100ml of Urea Lysis Buffer with a final formulation of 20mM HEPES, 9M urea, and 1X Phosphatase Inhibitor Cocktail when following the Directions for Use.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
To Purchase # 60055S
Product # Size Price
60055S
54.05 g $ 48