Western blot titration of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb (Biotinylated) #5373 and #7055 as the secondary antibody.
Affinity purified goat anti-biotin antibody is conjugated to calf intestinal alkaline phosphatase. This product has been optimized for the detection of biotinylated protein molecular weight standards in western blotting applications.
Store at -20°C.
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
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