Render Target: STATIC
Render Timestamp: 2024-10-11T10:50:44.466Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:17:34.428
Product last modified at: 2024-09-20T07:09:43.990Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) #5571

Filter:
  • WB

    Supporting Data

    REACTIVITY All
    SENSITIVITY
    MW (kDa)
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. This product has been optimized to detect biotinylated primary antibodies. HRP conjugated antibodies do not require incubation with a secondary antibody. *Do not mix this antibody in solution with any Anti-rabbit antibody. Anti-rabbit antibodies will cross react with this antibody and could result in decreased activity of both Anti-rabbit and Anti-biotin (D5A7) Rabbit mAb.

    Product Usage Information

    Recommended Antibody Dilutions:

    ELISA-peptide 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides and solid matrices.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a biotinylated protein.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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