Render Target: STATIC
Render Timestamp: 2024-10-11T10:14:20.844Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:22:50.507
Product last modified at: 2024-09-20T07:08:45.672Z
1% for the planet logo
PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody #51821

    Supporting Data

    REACTIVITY H
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    Affinity purified goat Anti-Human IgM, Fc5µ Fragment Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.

    Product Usage Information

    Reconstitute with 1.5 ml of dH20 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.

    Recommended Antibody Dilutions:

    ELISA (chromogenic substrates): 1:5K-1:100K

    Western Blotting (chromogenic substrates): 1:5K - 1:100K

    Western Blotting (ECL substrates): 1:10K - 1:200K

    Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

    Storage

    Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.

    Specificity / Sensitivity

    Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody detects the Fc5µ portion of human IgM heavy chain but does not react with human IgG or IgA, or the light chains of human immunoglobulins. This antibody has minimal cross-reaction with bovine serum proteins. It may cross-react with IgM from other species.

    Species Reactivity:

    Human

    Source / Purification

    Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgM. The Anti-Human IgM, Fc5µ Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.