|4412||Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)||
For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000. See protocol for more details.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.
This Cell Signaling Technology antibody was conjugated to DyLight® 488 fluorescent dye under optimal conditions and tested in-house for indirect flow cytometric and immunofluorescent analysis of human and mouse cells.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.
The Anti-rabbit IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG (H+L), F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion and chromatographic separation. The F(ab')2 Fragments were conjugated to DyLight® 488 under optimal conditions with an F/P ratio of 2-6.
Fluorescent Anti-rabbit IgG (H+L), F(ab')2 fragment is ideal for flow cytometry and immunofluorescence. This secondary antibody will detect rabbit antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. DRAQ5 is a registered trademark of Biostatus Limited. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.