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4315
Anti-rabbit IgG (H+L), F(ab')2 Fragment (DyLight® 488 Conjugate)
Secondary Antibodies

Anti-rabbit IgG (H+L), F(ab')2 Fragment (DyLight® 488 Conjugate) #4315

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# Product Name Application Reactivity
  • IF
  • F

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Goat 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody was conjugated to DyLight® 488 fluorescent dye under optimal conditions and tested in-house for indirect flow cytometric and immunofluorescent analysis of human and mouse cells.

Product Usage Information

For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000. See protocol for more details.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.

Specificity / Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.

Source / Purification

The Anti-rabbit IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG (H+L), F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion and chromatographic separation. The F(ab')2 Fragments were conjugated to DyLight® 488 under optimal conditions with an F/P ratio of 2-6.

Background

Fluorescent Anti-rabbit IgG (H+L), F(ab')2 fragment is ideal for flow cytometry and immunofluorescence. This secondary antibody will detect rabbit antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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