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Anti-Rabbit IgG (H+L), (PE Conjugate) #14705

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Inquiry Info. # 14705

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • F-Flow Cytometry 

    Product Information

    Product Description

    Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to R-phycoerythrin. This product has been optimized for use as a secondary antibody in flow applications. Excitation is 450-580 nm and peak emission is 590 nm.

    Product Usage Information

    The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.

    Storage

    Supplied in 10 mM HEPES, 150 mM NaCl, pH 7.4, 0.08% sodium azide. Store at 4°C. Protect from light for up to one year. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Anti-Rabbit IgG (H+L), (PE Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. Less than 1% cross-reactivity is observed with immunoglobulins from other species.

    Background

    This product has been optimized for use as a secondary antibody in flow cytometry applications. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
      For Research Use Only. Not for Use in Diagnostic Procedures.
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