Recommended Antibody Dilution:
Supplied in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease- Free) and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 19
Goat Anti-Mouse IgG2c, Fc gamma Specific Antibody (HRP Conjugate) is produced by immunizing goats with mouse IgG2c. The Anti-Mouse IgG2c, Fc gamma Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
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