Fluorescent and brightfield analysis of HCT 116 cells 48 hours post-transfection with either SignalSilence® Control siRNA (Cy5® Conjugate) (top panel, blue) or mock-transfected (bottom panel, no signal).Learn more about how we get our images
CST recommends transfection with 100 nM SignalSilence® Control siRNA (Cy5® Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 20 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 µl per well.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double-stranded RNA molecules into the cell. SignalSilence® Control siRNA (Cy5® Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is Cy5®-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalSilence is a registered trademark of Cell Signaling Technology, Inc. Cy5 is a registered trademark of GE Healthcare.
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|86921S||60 µl (0.5nmol)||$ 114.0|