CellSimple™ Cell Health Assay Kit #83323
Inquiry Info. # 83323
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Product Information
Product Description
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Background
Measures of cell viability and cytotoxicity are broadly used to study the effects of growth factors and cytokines, inhibitors and activators, and immune response signals. Calcein-AM is the acetomethoxyl form of calcein, a highly lipophilic, cell membrane permeable dye. Intracellular esterase activity converts the non-fluorescent Calcein-AM to the highly fluorescent Calcein, which is retained only within live cells (1,2). The DNA-binding agent PI is cell membrane impermeable and only enters dead cells or those with damaged cell membranes. Intracellular PI binds DNA and undergoes an approximate 40-fold enhancement in fluorescence intensity. As a result, live cells will produce a strong green fluorescence resulting from the conversion of Calcein-AM to Calcein, while dead cells produce a strong red fluorescence due to the presence of PI (3,4). These fluorescent signals can be detected using the Cell Health application on the CellSimple™ Cell Analyzer.
Alternate Names
Cell Simple; CellSimple
Limited Uses
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