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GLI1 (L42B10) Mouse mAb (Sepharose® Bead Conjugate) #11995

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Inquiry Info. # 11995

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 160
    Source/Isotype Mouse IgG1
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. GLI1 (L42B10) Mouse mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of GLI1. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GLI1 (L42B10) Mouse mAb #2643.
    MW (kDa) 160

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    GLI1 (L42B10) Mouse mAb (Sepharose® Bead Conjugate) recognizes endogenous levels of total GLI1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human GLI1 protein.

    Background

    GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).

    Alternate Names

    GLI; GLI family zinc finger 1; GLI-Kruppel family member GLI1; GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; PAPA8; PPD1; Zinc finger protein GLI1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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