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Nitrocellulose Sandwiches
WB & IP Reagents

Nitrocellulose Sandwiches #12369

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Store at room temperature in a cool and dry place away from light or heat sources. Nitrocellulose is extremely flammable and must be kept away from fire to avoid ignition. Stored appropriately, the product is stable for 12 months. Handle with gloves; nitrocellulose is highly absorbent and will bind proteins from skin contact which may reduce performance and decrease the quality of data.

Product Description

Nitrocellulose Membrane Dimensions: 80 x 90 mm, Pore Size: 0.2 μm, Binding Capacity: 115-125 μg IgG/cm2
Purity 100% pure nitrocellulose.


Nitrocellulose Sandwiches from Cell Signaling Technology® are pre-packaged sandwiches consisting of a precut nitrocellulose membrane and 2 sheets of 3 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists.
Nitrocellulose Sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose Sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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To Purchase # 12369
Cat. # Size Qty. Price
10 Pack  (10 western blots)