Immunoprecipitation of COX IV from HeLa cells using COX IV (4D11-B3-E8) Mouse mAb #11967 and Protein G Magnetic Beads. Western blot analysis was performed on the 10% input control (lane 1), IP pellet (lane 2) and Mouse (G3A1) mAb IgG1 Isotype Control #5415 (at matched concentration) (lane 3) using COX IV (4D11-B3-E8) Mouse mAb #11967.Learn more about how we get our images
Supplied in PBS, 0.1% BSA, 0.004% EDTA and 0.075% sodium azide. Store at 4°C. This product is stable for 12 months. Do not freeze, dry or centrifuge beads. This may cause irreversible aggregation and decreased binding capacity.
Protein G Magnetic Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein G is covalently coupled to a magnetic particle.
Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies. Beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017 or 12-Tube Magnetic Separation Rack #14654 which concentrates the beads to the side of the tube instead of the bottom. This eliminates centrifugation steps, minimizes sample loss, increases washing efficiency, and saves time.
The 1mL and 5mL size is enough material for 25 and 125 immunoprecipitations, respectively, when following our recommended protocol.
Bead Diameter: ~1.5 μm
Binding Capacity: > 0.2 μg Rabbit IgG/μl bead slurry
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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|70024L||5 ml (5 x 1ml)||$662.00.0|