Render Target: STATIC
Render Timestamp: 2024-11-12T10:53:18.358Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:16:15.893
Product last modified at: 2024-09-20T07:03:47.617Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody (Isotype Control)
PDP - Template ID: *******4b30f73
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) #3423

Filter:
  • IP

    Supporting Data

    Concentration 5000 bead
    SENSITIVITY Endogenous
    Source/Isotype Rabbit IgG
    Application Key:
    • IP-Immunoprecipitation 

    Product Information

    Product Description

    Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (whole molecule) from Cell Signaling Technology is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

    Product Usage Information

    Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as a control for rabbit IgG monoclonal antibodies.

    Background

    Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      XP is a registered trademark of Cell Signaling Technology, Inc.
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.