YAP (Yes-associated protein, YAP65) is a transcriptional co-activator that functions as the primary effector of the Hippo tumor suppressor pathway. Hippo signaling via YAP regulates tissue growth and organ size, in part by modulating the proliferative activity of stem and progenitor cells. YAP is a potent oncogene whose aberrant regulation can lead to tumorigenesis, and elevated YAP expression has been reported in many human tumor types. Research studies also suggest that YAP plays an important role in cell fate determination. In addition to its reported ability to promote epithelial-mesenchymal transition (1), YAP was shown to promote dedifferentiation of mature hepatocytes into hepatic progenitor cells, suggesting an important role for YAP and Hippo signaling in tissue regeneration (2).
We compared our YAP (D8H1X) XP® Rabbit mAb #14074 with 3 YAP antibodies from other companies and found that it performed as well or better in every application tested. See competitor comparison data below for individual applications.
Western blot analysis of extracts from YAP positive cells (A-204, A549, MCF7) and YAP negative cells (RL-7) using our YAP (D8H1X) XP® Rabbit mAb #14074, and three other antibodies at the recommended dilutions.
Summary: YAP (D8H1X) XP® Rabbit mAb #14074 demonstrates higher specificity and sensitivity than the other antibodies.
Of the antibodies we tested by western blot, only YAP (D8H1X) XP® Rabbit mAb #14074 and Antibody 2 rabbit monoclonal antibody are recommended for flow cytometry. We analyzed YAP positive A-204 cells (green) and YAP negative RL-7 cells (blue) using YAP (D8H1X) XP® Rabbit mAb #14074 and Antibody 2 at the recommended dilutions.
Summary: YAP (D8H1X) XP® Rabbit mAb #14074 demonstrates higher specificity than Antibody 2.
IHC analysis of paraffin-embedded YAP positive (PANC-1) and YAP negative (RL-7) cell pellets using YAP (D8H1X) XP® Rabbit mAb #14074 and antibodies from two other companies.
IHC analysis of paraffin-embedded breast carcinoma using YAP (D8H1X) XP® Rabbit mAb #14074 at the recommended dilution and a mouse monoclonal antibody from another company at the dilution that we determined to be optimal based on the cell pellet analysis.
Summary: Using cell pellets and paraffin-embedded breast carcinoma we demonstrate that YAP (D8H1X) XP® Rabbit mAb #14074 yields superior sensitivity and specificity in IHC assays than the antibodies from other companies.
IF-IC analysis of MCF10A and C2C12 cells using YAP (D8H1X) XP® Rabbit mAb #14074 and antibodies from two other companies.
Summary: YAP (D8H1X) XP® is specific in human and mouse cells while Antibody 3 demonstrates non-specific staining in rodent cells. YAP (D8H1X) XP® Rabbit mAb #14074 has higher specific activity than Antibody 1. In addition, Antibody 1 cross reacts strongly with a 50 kDa protein by western blot and it is possible that it also cross reacts with this protein by immunofluorescence.