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Caspase Cleavage Substrate Proteomics

The intrinsic and extrinsic apoptosis pathways involve a cascade of caspases. The human proteome contains thousands of known or putative caspase cleavage sites. The majority of caspase substrates are cleaved at an aspartic acid residue, generating fragments containing a carboxyl-terminal aspartate with a general DEXD motif with some variation.

PTMScan® technology for caspase cleavage proteomics uses a proprietary antibody to the DEXD motif to enrich caspase cleaved substrate peptides from trypsin digested samples prior to LC-MS/MS.

Caspase Sequencing

Caspase Cleaved Substrate Proteomics: The motif logo was generated from a PTMScan® LC-MS/MS experiment using 1044 nonredundant tryptic peptides with carboxy-terminal aspartates derived from HeLa cells treated with Staurosporine #9953 (1 μM, 3 hr) to induce apoptosis. Peptides were enriched using the PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit #12810. The logo represents the relative frequency of amino acids in each position leading up to the carboxy-terminal aspartate.

Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab Western Blot

Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab® Rabbit mAb mix #8698: Western blot analysis of NIH/3T3 and HeLa Cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +), using Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab® Rabbit mAb mix (upper) or GAPHD (D16H11) XP® Rabbit mAb #5174 as a loading control (lower).

Caspase Cleaved Substrate Proteomics Service

Target Description Motif Reference Data
Target Description Motif Reference Data
Cleaved Caspase Substrate DEXD