Phospho-Tyr antibody reacts broadly with Tyr-phosphorylated proteins. An induction in Tyr phosphorylation is observed upon stimulation of EGF receptor by treatment with EGF; EGFR mediated phosphorylation is inhibited by the tyrosine kinase inhibitor gefitinib.
This antibody reacts broadly with proteins containing phospho-Ser/Thr preceded by Lys/Arg at position -3. Phosphorylation of proteins containing this motif is induced by EGF treatment and inhibited by tyrosine kinase inhibitors, such as gefitinib.
This antibody reacts broadly with proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3. Phosphorylation of proteins containing this motif is induced by EGF treatment and inhibited by tyrosine kinase inhibitors, such as gefitinib.
This antibody reacts broadly with proteins phosphorylated on Thr residues in the context of sequences containing Arg or Lys at the +2 position. Phosphorylation of this motif is induced upon mitotic arrest with nocodazole treatment.
This antibody reacts broadly with proteins phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The data shown illustrates phosphoproteins containing this motif that are induced upon cell cycle arrest upon UV or nocodazole treatment. Phosphoproteins containing this motif are also significantly up-regulated by phorbol ester (PMA) and ionomycin treatment (data not shown).
This antibody has broad reactivity with proteins phosphorylated on Thr residues with proximal Pro residues at the -2 and +1 positions. CMGC family kinases including MAPKs and CDKs phosphorylate substrates containing this motif. Changes in phosphorylation of this motif are detected with nocodazole mediated mitotic arrest, as well as MEK/ERK signaling inhibition by U0126.
This antibody has broad reactivity with proteins phosphorylated on Ser residues with proximal Pro residues at the -2 and +1 positions. CMGC family kinases including MAPKs and CDKs phosphorylate substrates containing this motif. Changes in phosphorylation of this motif are detected with nocodazole mediated mitotic arrest, as well as MEK/ERK signaling inhibition by U0126.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr followed by Gln with a preference for Gly at the +2 position, a sequence motif found in substrates of kinases that include the ATM/ATR atypical protein kinases. Phosphorylation is stimulated by UV induced DNA damage and activation of ATM.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr followed by Gln, a sequence motif found in substrates of kinases that include the ATM/ATR atypical protein kinases. Phosphorylation detected with this antibody is stimulated by nocodazole mediated cell cycle arrest.
This antibody reacts broadly with proteins phosphorylated on Thr in sequences containing Pro at the +1 position, which is a motif recognized by MAPK and CDK family members. An increase in phosphorylation is observed with nocodazole treatment, consistent with the role of these kinases in cell cycle control.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr residues with Arg at the -3 position with a preference for Leu at the -5 position. This motif is found in phosphoproteins that include substrates of PKD family kinases.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr residues in sequences containing acidic residues Asp or Glu at +1 and +3 positions. Phosphoproteins containing this motif include substrates of CKII (casein kinase II). CKII family kinases are involved in cell differentiation, genotoxic stress response, and cell cycle regulation.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr residues with Arg at the -2 and -3 positions. This sequence motif is found frequently in substrates of PKA, as well as other AGC family kinases.
This antibody reacts broadly with proteins phosphorylated on Ser or Thr residues that are surrounded by hydrophobic amino acids, such as Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. Kinases that phosphorylate substrates containing such hydrophobic motifs are involved in diverse signaling pathways.
This antibody reacts broadly with proteins phosphorylated on Ser residues surrounded by Arg/Lys at -1 and +3 as well as Pro at +1 positions. CDK family kinases phosphorylate substrates containing this motif and phosphorylation is induced by nocodazole mediated cell cycle arrest.
