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PhosphoScan® Phosphorylation Proteomics

PTMScan Discovery Workflow

The reversible addition of phosphate groups by protein kinases can activate or inhibit protein function. Phosphorylation signaling cascades, for example the MAP kinase signaling pathway, are important for transmitting information from outside to inside the cell where it ultimately results in modulation of gene transcription. Aberrant phosphorylation is widespread in diseases including cancer, diabetes, and neurodegeneration. Therefore, identifying the phosphorylation status of proteins is crucial to understanding cellular processes in both health and disease.

Apply PhosphoScan® proteomics for

  • Pathway profiling
  • Biomarker discovery
  • Target validation

Why consider antibody enrichment for phosphorylation proteomics?

  • More focused discovery of phosphosites based on amino acid (S/T/Y) or motif of interest than IMAC.
  • PTMScan® methods and IMAC enrich different pools of phosphopeptides making them highly complementary. For the most complete dataset customers should use both types of enrichment. View data.

PTMScan Discovery Dot Matrix

Profiling tyrosine phosphorylation in MKN-45 cells. Peptides from MKN-45 cells treated for 2hr with DMSO (control), 1 μM SU11274 ,or 200 nM Staurosporine #9953 for were enriched using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit #8803 (above). In parallel, total protein levels were profiled by running unenriched material in LC-MS/MS (below). Peptides that decreased in abundance with SU11274 or Staurosporine are indicated in red, peptides that increased with treatment in green. A % CV histogram of analytical replicates for each enrichment is shown on the right with the median % CV indicated in blue.

PhosphoScan Services

Note: Custom mixes of Ser/Thr motif antibodies can be made for services. Please inquire.

Target Description Motif Reference Data
Target Description Motif Reference Data
14-3-3 Binding Motif (R/K)XX(s/t)XP  
Akt Substrate RXX(s/t) Mouse Liver | XLS | RAW
Akt Substrate RXRXX(s/t) Mouse Liver | XLS | RAW
AMPK Substrate LXRXX(s/t) Mouse Liver | XLS | RAW
ATM/ATR Substrate (s/t)Q Mouse Liver | XLS | RAW
ATM/ATR Substrate (s/t)QG Mouse Liver | XLS | RAW
CDK Substrate (K/R)(s/t)PX(K/R)  
CK2 Substrate (s/t)(D/E)X(D/E)  
MAPK/CDK Substrate PX(s/t)P, (s/t)PX(K/R) Mouse Liver | XLS | RAW
PDK1 Docking Motif (F/K)XX(F/Y)(s/t)F/Y) Mouse Liver | XLS | RAW
PKA Substrate (K/R)(K/R)X(s/t) Mouse Liver | XLS | RAW
PKC Substrate (K/R)X(s/t)X(K/R) Mouse Liver | XLS | RAW
PKD Substrate LXRXX(s/t)  
PLK Binding Motif S(s/t)P Mouse Liver | XLS | RAW
tP Motif (s/t)P, (s/t)PP  
tPE Motif (s/t)PE Mouse Liver | XLS | RAW
tXR/tPR Motif (s/t)XR, (S/t)PR Mouse Liver | XLS | RAW
Phospho-Tyrosine (pY-1000) y Mouse Brain | XLS | RAW