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32793
IFN-γ (XMG1.2) Rat mAb (APC Conjugate)
Antibody Conjugates

Monoclonal Antibody - IFN-γ (XMG1.2) Rat mAb (APC Conjugate), UniProt ID P01580, Entrez ID 15978 #32793

APPLICATIONS

REACTIVITY SENSITIVITY MW (kDa) Isotype
M Endogenous Rat IgG1, kappa
Flow Cytometry - IFN-γ (XMG1.2) Rat mAb (APC Conjugate)

Flow cytometric analysis of live mouse splenocytes, untreated (left column) or treated with 1X Cell Stimulation Cocktail (with protein transport inhibitors) (500X) (right column), using IFN-γ (XMG1.2) Rat mAb (APC Conjugate) (top row) or concentration-matched Rat Isotype Control (APC Conjugate) (bottom row), and co-stained with CD3 (17A2) Rat mAb (FITC Conjugate) #86603.

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Flow Cytometry using the FoxP3/Transcription Factor Fixation/Permeabilization Kit

This antibody has been validated for Flow Cytometry using the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. Please refer to the protocol provided with the kit for detailed instructions.

Protocol Id: 1804

For optimal flow cytometry results, we recommend 0.06 μg of antibody per test.

Application Dilutions
Flow Cytometry 1:300
Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

IFN-γ (XMG1.2) Rat mAb (APC Conjugate) recognizes endogenous levels of total IFN-γ protein. This antibody detects an epitope within the intracellular domain.

Species Reactivity:

Mouse

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

  1. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
  2. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
  3. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
  4. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.
Entrez-Gene Id
15978
Swiss-Prot Acc.
P01580
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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