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12185
PhosphoPlus® PERK (Thr980) Antibody Duet
Primary Antibodies

PhosphoPlus® PERK (Thr980) Antibody Duet, UniProt ID Q9NZJ5, Entrez ID 13666 #12185

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Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.

Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.

To Purchase # 12185S

Kit Usage Information

Protocols

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

  1. Harding, H. et al. (1999) Nature 397, 271-274.
  2. Harding, H. et al. (2000) Mol. Cell 5, 897-904.
  3. Harding, H. et al. (2000) Mol. Cell 5, 897-904.
  4. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.