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Monkey Protein Phosphatase Type 1 Complex

$303
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Immunohistochemistry (Paraffin), Western Blotting

Background: Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

$260
100 µl
APPLICATIONS
REACTIVITY
D. melanogaster, Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: SHOC2 is a scaffolding protein that harbors multiple leucine-rich repeats in tandem and is an upstream positive regulator of growth factor-dependent MAPK/ERK signaling. Research studies have demonstrated that SHOC2 forms a complex with the catalytic subunit of the PP1 phosphatase and M-Ras, and this complex drives activation of Raf-ERK signaling in response to mitogenic growth factors (1). SHOC2 has also been shown to cross-talk with and activate the PI3K/Akt signaling axis through its interaction with the p110α catalytic subunit of PI3K (2). As a positive regulator of ERK and PI3K/Akt signaling cascades, SHOC2 has been implicated in the regulation of several oncogenic cellular processes such as cell motility, invasion, and metastasis (2). A mutation in SHOC2 that introduces an N-terminal myristoylation site, promotes aberrant membrane targeting of SHOC2, hyperactive MAPK/ERK signaling, and Noonan-like syndrome (3).

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Immunoprecipitation, Western Blotting

Background: NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

$260
100 µl
APPLICATIONS
REACTIVITY
Hamster, Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).