Sonication is a popular choice for preparing chromatin for ChIP. However, most protocols require subjecting the chromatin to harsh, denaturing conditions (i.e., high heat, detergent, and shearing force) that can damage both antibody epitopes and the integrity of the chromatin.
CST provides multiple options for avoiding the problems of traditional sonication protocols:
The CST SimpleChIP kits for sonication or enzymatic digestion, work as well as traditional sonication for assessing histones, and both outperform traditional sonication when assessing either transcription factors or cofactors.
Chromatin was cross-linked and then fragmented with micrococcal nuclease (CST-enzymatic; blue track), CST sonication buffer (red track) or traditional sonication buffer (green track) and immunoprecipitated using the indicated antibodies. DNA was prepared for sequencing as outlined in the protocol for the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005.
|#56383||SimpleChIP® Plus Sonication Chromatin IP Kit (Magnetic Beads)||ChIP, ChIP-seq||Cells and Tissue|
|#9005||SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)||ChIP, ChIP-seq||Cells and Tissue|
|#9003||SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)||ChIP, ChIP-seq||Cells|
|#9004||SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)||ChIP||Cells and Tissue|
|#9002||SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)||ChIP||Cells|
|#81804||SimpleChIP® Sonication Cell and Nuclear Lysis Buffers||ChIP, ChIP-seq||Cells and Tissue|
|#14282||SimpleChIP® Enzymatic Cell Lysis Buffers A & B||ChIP, ChIP-seq||Cells and Tissue|
|#14231||SimpleChIP® Chromatin IP Buffers||ChIP, ChIP-seq||Cells and Tissue|
|#14209||SimpleChIP® DNA Purification Buffers and Spin Columns||ChIP, ChIP-seq||Cells and Tissue|