Advantages of Intracellular Flow Cytometry
- Concurrent detection of proteins associated with surface, cytosolic and nuclear compartments
Direct correlation of phenotype with:
- Intracellular protein expression
- Functional output, e.g., cytokine release, production of transcription factors
- Study of signaling events on a single cell basis
- Study of the mechanism of action of pathway activation/inhibition for cells in suspension
- Quantitative assessment of intracellular protein expression