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Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.
Distinguish living cells from dead or dying cells with viability dyes.
Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research
Trademark information appearing on Cell Signaling Technology website.
What Assay should you be for Halloween? Western Blot, IHC, IF, Flow Cytometry, ChIP, or ELISA...
Learn about host-based and dye-conjugated methods for IF multiplexing, as well as a sequential labeling strategy that works with indirect detection.
Mutations in cell death pathways, such as apoptosis, mitophagy, necroptosis, and autophagy, contribute to neuronal cell death and the progression of neurodegenerative diseases.
Validation for IF antibodies at CST Cell Signaling Technology
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.
We’ve put together a starter’s guide on the cellular mechanisms that drive neurodegeneration in diseases such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis, and Huntington’s disease.
The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Epigenetic regulation, including aberrant DNA methylation and histone modifications, have been linked to Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, and amyotrophic lateral sclerosis.
DIY conjugation kits let researchers label an antibody themselves but lengthy protocols, poor conjugate quality and low yield cause inconsistent results.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Abnormal cell-cell communication, for example disrupted presynaptic input, as well as disrupted intracellular signaling contribute to the pathogenesis of neurodegenerative disease.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
The three main purposes of metabolism are the conversion of food to energy; the conversion of nutrients to proteins, carbohydrates, lipids, and nucleic acids; and the elimination of nitrogenous wastes. There is a strong correlation between metabolic chan
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spectra of DRAQ5 and DyLight 680 overlap, and the signal produced by each dye cannot be distinguished.
Modulation of CNS function occurs through synaptic neurotransmission, which is the signaling from the axon terminal of one neuron to the dendrites of another via molecules called neurotransmitters.
Custom-build antibody conjugates that match your assay requirements and deliver superior performance, and choose from a broad catalog of labels.
Cellular senescence is characterized by irreversible cell-cycle arrest in combination with a distinct secretory phenotype and expanded lysosomes in response to stress. Senescent cells accumulate in tissues during aging, and various markers of senescence
A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).
Reggie Prioli, Associate Director of Conjugation at CST®, has been conjugating antibodies at CST since 2001. Let’s find out why!
Tau is a microtubule-associated protein found in neurons, and its function is to stabilize axonal microtubules. Hyperphosphorylated tau is a hallmark of several neurodegenerative diseases including Alzheimer’s disease.
Read about the project to validate CST antibodies for spatial biology research on the Leica Cell DIVE, which can image 60+ biomarkers in one tissue sample.