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Expert-reviewed interactive dopamine signaling pathway providing a current overview of Dopamine in Parkinson's Disease. Learn more here.
Ensure equal sample loading with loading control antibodies from CST.
Western Blotting troubleshooting guide for easy to solve high and low background issues.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.
Learn about BCR signaling and how it can lead to changes in cell metabolism, gene expression, and cytoskeletal organization. Click here.
Explore the NFkB pathway and its impact on inflammation and immune responses. Click here to learn more about this signaling pathway.
A ChIP experiment uses 4M cells per reaction while a CUT&RUN assay only uses 5K to 100K starting cells, so it is expected to see a lower DNA yield from a CUT&RUN assay than from ChIP experiments. We have found that digestion at 4C can increase not only the signal strength but also the DNA yield. DNA purification with phenol/chloroform can also increase the DNA yield but it also can increase the background, so we do not recommend it. Always start with more cells if you can, and make sure that your cells are healthy. Please note that a high specificity of the DNA sample and not a high DNA yield is what you need to pursue for a good result.
Discover the PI3K Akt pathway and its crucial role in cell growth and survival. Click here to learn more & gain insights into this important signaling cascade
For IHC, we recommend combining the peptide and the primary antibody at a 10:1 ratio (µg : µg) in the antibody diluent. Incubate the peptide/antibody mixture for a minimum of 30 minutes prior to adding the entire volume to the slide. The recommended antibody dilution can be found on the product's web page.
An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity.
Designing or troubleshooting flow cytometry experiments to analyze intracellular signaling? Here’s what you need to know.
Cell Signaling Technology announces the launch of SignalStar™ Multiplex IHC, a flexible spatial biology solution for up to 8-plex amplification in two days.
Protein enrichment by immunoprecipitation is frequently carried out prior to analysis. In this Tech Tip, Sarah presents considerations for choosing antibodies and beads for IP.
Nov 2008, Renewal of a research agreement with Ortho-Biotech Oncology Research & Development, under which CST will employ its patented PhosphoScan® and PTMScan® technologies in research aimed at biomarker discovery and validation for multiple enzyme target classes and lead small-molecule inhibitors.
Get information on ordering or contacting Cell Signaling Technology. CST is happy to answer your questions. Select the team you need to contact.
CiteAb Names Cell Signaling Technology, "Antibody Company of the Year" and "Researchers’ Choice"
Cell Signaling Technology (CST), a leading provider of antibodies, kits, and services, was honored with the 2017 CiteAb Award for &quoPTM Antibody Company of the Year" and for a third year in a row with the award for "Researchers’ Choice."
CiteAb Names Cell Signaling Technology "Antibody Company of the Year" and "Antibody Company Succeeding in Cancer Biology"