Do you have a peptide blocking protocol for IHC?

For IHC, we recommend combining the peptide and the primary antibody at a 10:1 ratio (µg : µg) in the antibody diluent. Incubate the peptide/antibody mixture for a minimum of 30 minutes prior to adding the entire volume to the slide. The recommended antibody dilution can be found on the product's web page.

 

Last updated: September 12, 2024