ChIP-seq Validated Antibodies
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment. CST is validating recombinant rabbit monoclonal antibodies for ChIP-seq and we provide sample data for all validated products on the product pages. These high quality antibodies provide better lot-to-lot consistency and target specificity making them the ideal choice for your ChIP-seq experiments.
3D video animation of histone modifications and their effects on chromatin activation
Benefits of Enzyme-based Chromatin Digestion (Application Overviews)
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
ChIP Validated Antibodies and Primer Pairs (Application Overviews)
SimpleChIP® Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.
ChIP-Sequencing performed using the SimpleChIP® Kit (Application Overviews)
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
Chromatin / Epigenetics Resources (Pathway Diagram)
Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.
Chromatin Immunoprecipitation (Application Overviews)
Comprehensive SimpleChIP® kits and ChIP validated antibodies from CST and validated ChIP reagents (primer pairs, Protein G agarose and magnetic beads
Chromatin Immunoprecipitation Technical Support (Application Overviews)
Detailed technical support information to support ChIP experimental success. LInk to CST's comprehensive ChIP protocol.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
Chromatin IP Frequently Asked Questions (Application Overviews)
Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
Histone Modification Table (Table Resources)
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
In-Cell Western™ (Application Overviews)
In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.
Overview of Chromatin IP Assay Methodology (Application Overviews)
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.
Product Shelf-Life (Product Shelflife Table)
A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).
SimpleChIP® Assay Kits (Product Type Overviews)
Assay Kits from CST contain ChIP validated antibodies and control PCR primers to assay for pluripotency markers or epigenetic mark status in human or mouse samples.
SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Kits (Product Type Overviews)
CST provides multiple options for avoiding the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions that can damage both antibody epitopes and the integrity of the chromatin.
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.