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SimpleChIP® Enzymatic ChIP Kits

SimpleChIP® Enzymatic Chromatin IP Kits from Cell Signaling Technology are optimized to detect endogenous levels of protein-DNA interactions in cultured cells while SimpleChIP® Plus Chromatin IP Kits are optimized for cultured cells and tissue samples. These kits contain all reagents necessary to perform enzymatic digestion-based chromatin immunoprecipitation (ChIP) experiments and include positive and negative controls that ensure confidence in your results. These kits are available with either Protein G agarose or Protein G magnetic beads.
 
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Pathway Protocol

An Introduction to Epigenetics

3D video animation of histone modifications and their effects on chromatin activation

Benefits of Enzyme-based Chromatin Digestion (Application Overviews)

The benefits of using micrococcal nuclease to digest chromatin compared to sonication.

ChIP Validated Antibodies and Primer Pairs (Application Overviews)

SimpleChIP® Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

ChIP-Sequencing performed using the SimpleChIP® Kit (Application Overviews)

Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.

Chromatin / Epigenetics Resources (Pathway Diagram)

Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, pathway diagrams, and technical resources for epigenetics research.

Chromatin Immunoprecipitation (Application Overviews)

Comprehensive SimpleChIP® kits and ChIP validated antibodies from CST and validated ChIP reagents (primer pairs, Protein G agarose and magnetic beads

Chromatin Immunoprecipitation Technical Support (Application Overviews)

Detailed technical support information to support ChIP experimental success. LInk to CST's comprehensive ChIP protocol.

Chromatin IP Frequently Asked Questions (Application Overviews)

Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

Comparison of SimpleChIP® Plus Chromatin IP Kits with a Kit from Another Supplier (Application Overviews)

Comparison of SimpleChIP® Plus Chromatin IP Kits with a Kit from another supplier using tissue samples. Data shows the advantage of enzymatic digestion vs sonication.

Enzymatic Chromatin Immunoprecipitation Troubleshooting Guide (Protocols)

Enzymatic Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.

Histone Modification Table (Table Resources)

The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.

In-Cell Western™ (Application Overviews)

In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.

Overview of Chromatin IP Assay Methodology (Application Overviews)

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.

Product Shelf-Life (Product Shelflife Table)

A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).

SimpleChIP® Assay Kits (Product Type Overviews)

Assay Kits from CST contain ChIP validated antibodies and control PCR primers to assay for pluripotency markers or epigenetic mark status in human or mouse samples.

SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads) (Protocols)

SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.

SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads) (Protocols)

SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.

SimpleChIP® Kits (Product Type Overviews)

CST provides multiple options for avoiding the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions that can damage both antibody epitopes and the integrity of the chromatin.

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads) (Protocols)

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads) (Protocols)

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.

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