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Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
An enzyme-linked immunosorbent assay (ELISA), or enzyme immunoassay (EIA), utilizes the specific interaction between an antibody and an antigen to selectively bind a substance that is most commonly peptides, proteins, antibodies, or hormones.
The following three types of analysis are used to analyze results from ELISA assays: qualitative, semiquantitative, and quantitative.
CST ELISA kits and reagents let you measure key cellular signaling nodes and other cellular target proteins using a wide selection of sandwich antibody assays.
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
FastScan ELISA kits detect key signaling proteins using a solution-based assay format and are validated to ensure reproducible results across lots and users.
HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.
Optimization is critical to obtaining reliable ELISA results. The design and development of an ELISA assay depends on the various components, including capture antibody, blocking buffer, diluent, sample concentration, and detection antibody
PathScan ELISA kits detect endogenous levels of key signaling proteins using a traditional sandwich-based assay format in phospho-specific or total protein options.
PathScan® Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.
Protocol for PathScan® Chemiluminescent Sandwich ELISA (for kits with Lyophilized Antibodies)
PathScan® Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.
PathScan® Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
PathScan® Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.
Several types of ELISAs have been developed: direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA, multiplex ELISA. The types of ELISAs are categorized based on the type of antibodies and enzymes used to bind the antigen of interest.