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Compare Cell Signaling Technology's Akt antibodies against one another to determine optimal applications
Overview of PI3K/Akt signaling pathways, antibodies and related reagents, interactive pathway diagrams, and other technical resources.
Phospho-Akt product and resources including product citations, educational videos and pathways, product selection tools and comparison data from Cell Signaling Technology
The Phospho-Akt (Thr308) Antibody #9275 will recognize all 3 isoforms of Akt: Ak1, Akt2, and Akt3.
The Akt Binding Partners Table provides a list of proteins that bind to Akt along with their effects, activities, and corresponding PubMed reference(s).
Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
Our Akt Antibody #9272 detects all 3 isoforms of Akt: Ak1, Akt2, and Akt3.
Discover the PI3K Akt pathway and its crucial role in cell growth and survival. Click here to learn more & gain insights into this important signaling cascade
A Hallmark of Cancer, sustaining proliferative signaling is used by cancer cells to stimulate growth and relies on Akt, MAPK/Erk, and MTOR pathways.
It is not uncommon for Akt to appear as a doublet on a western blot. This double banding is due to differential phosphorylation of the different Akt isoforms. If a doublet is seen with a phospho-specific Akt antibody, it should also be seen with a total Akt antibody. From referencing our in-house testing, the doublet is more commonly seen with mouse samples.
Listing of reference table resources for cellular regulation and signaling research
Video Tutorial on PI3K / Akt Signaling created by CST on upstream stimuli and downstream effects on cellular events (autophagy, mTOR)
Synopsis of CST scientists' publication in the journal Science Signaling describing phosphorylation patterns associated with non-small cell lung cancer (NSCLC).
Expert-reviewed interactive pathway providing a current overview of Inhibition of Apoptosis Signaling.
Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue
"PI3K/Akt signaling is close to my heart because certain diseases that have cropped up in my family are linked to specific proteins in this pathway."
Our Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 detects over-expressed levels of PKCalpha and beta. It also detects endogenous levels of PKC in Jurkat + CalA extract and possibly weakly in other endogenous models tested. Our Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 and Phospho-Akt (Thr308) Antibody #9275 detect over-expressed levels of PKCalpha and beta, but do not detect endogenous PKC. Our Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 detects over-expressed levels of PKCalpha and beta and may also weakly detect endogenous PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Thr308) (D25E6) XP Rabbit mAb #13038 does not detect over-expressed PKC and is the best choice of the Phospho-Akt (Thr308) antibodies if this is a concern.
Video tutorials illustrate the science behind cellular processes and signaling pathways.
Publications featuring Akt antibodies from Cell Signaling Technology.
Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.
Find Pathways and Diagrams By Disease Area
The three main purposes of metabolism are the conversion of food to energy; the conversion of nutrients to proteins, carbohydrates, lipids, and nucleic acids; and the elimination of nitrogenous wastes. There is a strong correlation between metabolic chan
Discover the insulin signaling pathway and its role in glucose metabolism & diabetes. Learn more about insulin action here.
CST antibodies have been validated for Simple Western, the only platform on the market that provides a fully automated western blotting workflow.
Identifies and quantifies up to many hundreds of critical signaling nodes across pathways involved in cancer biology, immune cell signaling, and more.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
Products and Related Resources for Fibrosis SARS-CoV-2 Research
A demonstration of the perils of deviating from Cell Signaling Technology's recommended protocols.
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
This video introduces PTMScan technology, a platform for the enrichment of post-translational modifications (PTMs) in mass spectrometry-based proteomics studies.
