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Our Ubiquitin (P4D1) Mouse mAb #3936 weakly detects free Ubiquitin. The Ubiquitin signal can be increased by using a semi-dry-transfer and autoclaving (30 min. wet cycle) or boiling the membrane for 30 minutes after transfer.
Learn about antibody diversity, host species, isotypes, and how antibodies are developed for research, clinical diagnostics, and therapeutic uses.
Western Blotting troubleshooting guide for easy to solve high and low background issues.
Study the intricate machinery of the apoptosis signaling pathway. Click here to learn more and gain insights into programmed cell death mechanisms.
In this webinar, the speakers will explore how tumors exploit immune modulatory mechanisms to generate and thrive in their own immunosuppressive microenvironment, and explain how PD-1 immunotherapy stops certain cancers from turning off the anticancer immune response.
It’s the start of another exciting year of Lab Expectation articles. What can you expect from us in 2021?
Multimers are likely to demonstrate higher apparent affinity due to avidity effects. However, an exact recommendation for a competition assay would depend on other assay parameters, specifically assay format (in-tandem, classical sandwich, or premix), tags present on other proteins being used, and detection method.If you want to immobilize ACE2, we recommend using the Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 due to the ease of immobilization by protein A or anti-Fc. If you want ACE2 in solution, we recommend using the Human ACE2 (multimeric) (18-652) Recombinant Protein #85054 if you are immobilizing other proteins by protein A or anti-Fc. This is because the multimeric protein does not cross-react at protein A or anti-Fc surfaces. If you would like ACE2 in solution but are not using protein A or anti-Fc surfaces for immobilization, either protein should work well. Finally, if you want the most potential avidity in solution, then the multimeric protein is preferable.
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
This post explores common T cell types, their roles in modulating immune cell response, and T cell activation via interaction with cell surface receptors.
Explore the complex inflammation signaling pathways. Click here to learn more and understand the molecular basis of inflammation.
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clones. The p-Tyr designations, including “p-Tyr-100” and “p-Tyr-1000,” are a naming convention to allow customers to readily differentiate between the multiple products that we offer against this motif. The designations are not meant to suggest differences in quality or performance. In general, #8954 is a stronger antibody compared to #9411, and using our PTMScan® technology, we have found that #8954 detects more unique phosphorylated peptides than #9411. Both products are highly specific to phosphorylated tyrosine residues.
Expert-reviewed interactive neuroscience pathways providing current overviews of Parkinson's and Alzheimer's Disease signaling, as well as links to products from CST.
The nuclear receptor signaling plays important roles in cell differentiation, proliferation, metabolism, and other physiological processes. Click here.
A scientific resource for the BEACH protein domain containing information on structure, function, and phospholipid binding.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.
A scientific resource for the ANK protein domain containing information on structure, function, and binding partners.
Cell behavior is a tightly regulated process where various signals work together to elicit a specific response. Unraveling these mechanisms allows us to understand how cells are regulated in a normal state.
Short videos from CST highlighting our optimized western blotting protocol and troubleshooting guide to diagnose issues and recommend solutions.
Grant application guidelines and project description criteria for funding from CST.
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We’re always trying find ways to make life simpler for those in the lab. So, we’ve developed FastScan™ ELISA. It helps you get to your results quicker.
No matter your research area, we’ve all needed to answer this deceptively easy question: how much of a particular protein is present in my samples?
Publications featuring Akt antibodies from Cell Signaling Technology.
Mastering Phospho-Flow: Immune Cell Activation Webinar
An interactive 3D molecular model depicting the cellular landscape of a nucleus and its various protein subsets including DNA Damage, Replication, Transcription, etc.
A scientific resource for the ARM protein domain containing information on structure, function, and domain binding.
CST's Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final results.
CST's Western Blotting (WB) Troubleshooting Guide Video provides tips to help you diagnose experimental issues and offers recommendations to solve the problems.
Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.
Companion reagents influence the binding of a primary antibody to its epitope dramatically affecting results. Learn IHC troubleshooting tips in this series