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Our testing on recombinant proteins indicates that the Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 detects JNK1 (p46) and JNK2 (p54) well and JNK3 (p49) only weakly. Bands observed at 46kDa and 54kDa can be a mix of either JNK1 or JNK2, as each protein has multiple isoforms that run at these sizes.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.
Our Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251 recognizes all three isoforms of JNK (i.e., JNK1, JNK2, and JNK3) when dually phosphorylated.
Overview of MAP Kinase signaling pathways, antibodies and related reagents, interactive pathway diagrams, and other technical resources.
Expert-reviewed interactive pathway providing a current overview of SAPK/JNK Signaling.
The transcriptional activity of c-Jun is regulated by SAPK/JNK phosphorylation at Ser63 and Ser73. The signaling pathway can be found at https://www.cellsignal.com/contents/science-pathway-research-mapk-signaling/sapk-jnk-signaling-pathway/pathways-mapk-sapk. Cellular stressors, such as UV radiation, inflammatory cytokines, or ceramide, activate Rho-family GTPases and subsequent MAPKKK (Map Kinase Kinase Kinase) pathways. MAPKKKs can activate MKK4/7, which directly phosphorylate and activate SAPK/JNK. Upon translocation to the nucleus, SAPK/JNK can activate c-Jun and other transcription factors. Proteins generally undergo a phosphorylation dependent mobility shift (PDMS) in SDS-PAGE. This shift can vary from target to target, and even with different activation events on the same target. Recent experiments suggest this is due to the additional negative charge conferred by phosphorylation sites disrupting protein binding to SDS (see https://www.researchgate.net/publication/264031559_Phosphorylation-Depe…
As a new antibody is developed, Cell Signaling Technology scientists test the antibody’s stability and activity
Learn more about commonly used chemical modulators to study cellular physiology and biology.
Expert-reviewed interactive pathway providing a current overview of Death Receptor Signaling.
Learn about BCR signaling and how it can lead to changes in cell metabolism, gene expression, and cytoskeletal organization. Click here.
Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling.
Identifies and quantifies up to many hundreds of critical signaling nodes across pathways involved in cancer biology, immune cell signaling, and more.
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
Interactive p38 MAPK pathway. Learn how p38 MAPK is involved in regulation of HSP27, MAPKAPK-2, MAPKAPK-3, & several transcription factors.
Expert-reviewed interactive dopamine signaling pathway providing a current overview of Dopamine in Parkinson's Disease. Learn more here.
Expert-reviewed interactive pathway providing a current overview of TLR signaling.
Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.
The Akt Binding Partners Table provides a list of proteins that bind to Akt along with their effects, activities, and corresponding PubMed reference(s).
Discover the TGF Beta signaling pathway and impact on cell growth & tissue homeostasis. Learn here the mechanisms behind this vital signaling cascade.
A Hallmark of Cancer, sustaining proliferative signaling is used by cancer cells to stimulate growth and relies on Akt, MAPK/Erk, and MTOR pathways.
Cell Signaling Technology pathways by research area
Understanding how TLR signaling is activated by molecular patterns is relevant not only to their role in combating infection but also in cancer.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
A number of growth factors and cytokines can induce an Epithelial-Mesenchymal Transition (EMT) in tumor cells.
Interact with this CAR-T signaling pathway to learn how the combination of extracellular and intracellular domains, including CD8, CD28, 4-1BB, OX40, or CD27, exert antitumor effects.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.