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Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2BPMID: 24913553 (https://pubmed.ncbi.nlm.nih.gov/24913553/) Figure 1D
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
Discover the mTOR pathway and its role in cellular growth and metabolism. Click here to explore the intricacies of this vital signaling pathway.
Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
mTOR is the catalytic subunit of two functionally distinct complexes, mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTORC1 is composed of mTOR, Raptor, mLST8/GL, PRAS40 and DEPTOR. mTORC2, on the other hand, is composed of mTOR, Rictor, GßL, and mSIN1. Our total mTOR antibodies (#2983, #2972, and #4517) recognize total mTOR kinase in both complexes.
Resources for the study of Translational Control, Protein Synthesis, and RNA Regulation including antibodies and associated reagents and interactive pathway diagrams.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.
The root material used to make SignalStain® Boost IHC Detection Reagent (HRP, Rat) #72838 is goat anti-rat IgG. However, in various formats (biotinylated, etc.) this root material has been shown to have a range of cross-reactivity with rat IgM from 12% to 50%.
The percent cross-reactivity of Anti-mouse IgG, HRP-linked Antibody #7076 is 6% for rat IgG and <1.0% for rat IgM, human IgG, and human IgM.
The reactivity of the Anti-rat IgG, HRP-linked Antibody #7077 to IgG subclasses has not been tested, however antibodies to rat IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared. Cross-reactivity with mouse serum has been minimized with affinity procedures.
Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Ser80 in human, which corresponds to Ser79 in mouse and rat. They also recognize ACC-beta only when phosphorylated at Ser222 in human, corresponding to Ser212 in mouse and Ser218 in rat.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
A scientific resource for the SNARE protein domain containing information on structure, function, and binding to mediate protein-protein interactions in vesicle trafficking.
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
The Ras Mouse mAb #8832 included in the Active Ras Detection Kit #8821 will detect H, K, and N Ras. It cross-reacts with human, mouse, and rat Ras.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Modulation of CNS function occurs through synaptic neurotransmission, which is the signaling from the axon terminal of one neuron to the dendrites of another via molecules called neurotransmitters.
When planning your western blot experiment, there are a few things to keep in mind.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
Identify microglia by using these antibodies, which are designed to detect protein biomarkers specifically expressed within these cells.
The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Our Fc Block CD16/CD32 (2.4G2) Rat mAb #88280 binds to the Fc receptor on the surface of live cells and blocks the interaction of the Fc receptor with the Fc domain of antibodies. Preventing this interaction ensures that your antibodies will bind only to their intended target, rather than being sequestered by Fc receptors.
It has been determined that our Anti-rabbit IgG, HRP-linked Antibody #7074 has the following low cross-reactivities with species other than rabbit: