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Expert-reviewed interactive pathway providing a current overview of Regulation of eIF4E and p70 S6K.
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 and Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858. For this application, the Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 is preferred.
We have several antibodies for detecting ribosomal protein S6 (rpS6) that are validated for IF-IC with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse mAb #2317. Both of these antibodies are suitable for immunofluorescence in mouse cells.
The three main purposes of metabolism are the conversion of food to energy; the conversion of nutrients to proteins, carbohydrates, lipids, and nucleic acids; and the elimination of nitrogenous wastes. There is a strong correlation between metabolic chan
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211, Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858, Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857, Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856, and Phospho-S6 Ribosomal Protein (Ser235/236) (E2R1O) Mouse mAb #62016. All of these antibodies are suitable for immunofluorescence in mouse cells.
We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse mAb #2317. For this application, S6 Ribosomal Protein (5G10) Rabbit mAb #2217 is preferred, as S6 Ribosomal Protein (54D2) Mouse mAb #2317 could be problematic due to mouse on mouse (MOM) background staining.
Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.
Antibody 5G10, which targets S6 ribosomal proteins, can be used as a control for formaldehyde fixation conditions and sample quality in IF experiments.
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse mAb #2317. These antibodies perform similarly for IHC-P with human samples, therefore, there is no preference for this application.
Resources for the study of Translational Control, Protein Synthesis, and RNA Regulation including antibodies and associated reagents and interactive pathway diagrams.
Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
This video introduces PTMScan technology, a platform for the enrichment of post-translational modifications (PTMs) in mass spectrometry-based proteomics studies.
We have tested both the S6 Ribosomal Protein (54D2) Mouse mAb #2317 and the S6 Ribosomal Protein (5G10) Rabbit mAb #2217 for IP; however, both failed to meet our standards for IP recommendation. While each antibody did appear to pull down at 1:50, the signal was equivalent in the IgG control lane therefore we do not recommend using either product for IP.
A video tutorial illustrating the methodology behind PTMScan Technology and an example of its use in a recent phosphoproteomic study authored by scientists from CST.
Synopsis of CST scientists' publication in the journal Science Signaling describing phosphorylation patterns associated with non-small cell lung cancer (NSCLC).
Cell Signaling Technology pathways by research area
Case Study 2: Exceptional Sensitivity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
To perform intracellular flow cytometry, fixation and permeabilization of cells is required to allow for antibody penetration and subsequent binding to targets without disrupting cellular morphology.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
Abnormal cell-cell communication, for example disrupted presynaptic input, as well as disrupted intracellular signaling contribute to the pathogenesis of neurodegenerative disease.
We originally recommended milk as the western blot diluent for our Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit mAb #97596. However, after additional testing, we found that dilution in BSA showed stronger signal by western blot. Since that time, the diluent has been switched back to milk because we have been seeing a potentially non-specific band just above the p85 band in non-human samples. Milk appears to reduce the appearance of this band without decreasing target signal. We have decided reducing the background is more important than the slight increase in signal.
Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling.
Scientists used around 30 different Cell Signaling Technology® antibodies to characterize the Ras GTPase-activating protein-binding proteins G3BP1 and G3BP2.
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
Learn more about how intracellular flow cytometry can be used to measure unique signaling events to do with apoptosis, pluripotency status and epigenetic activity.
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
Get the reagents and supplies for immunohistochemistry (IHC) protocols including antibodies, diluent, blocking buffer, chromogens, and controls
Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be included to demonstrate:
Every IF staining experiment we perform at CST includes the following elements:
1. S6 Ribosomal Protein (5G10) Rabbit mAb #2217 as a robust readout for both quality of fixation/p…
