Why was the diluent changed for your Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit mAb #97596?

We originally recommended milk as the western blot diluent for our Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit mAb #97596. However, after additional testing, we found that dilution in BSA showed stronger signal by western blot. Since that time, the diluent has been switched back to milk because we have been seeing a potentially non-specific band just above the p85 band in non-human samples. Milk appears to reduce the appearance of this band without decreasing target signal. We have decided reducing the background is more important than the slight increase in signal.

Last updated: September 12, 2024