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Cancer cells can resist death. They evade apoptosis, the mechanism that programs cell death once cells become damaged. Normally, apoptosis helps keep an organism healthy through growth and development, but cancer cells do not follow this process, no matter how abnormally they grow.
An outline and examples of the steps performed at Cell Signaling Technology to validate target specificity of an antibody for use in immunohistochemical analysis.
Obtain high-quality data using CST antibodies to examineRNA binding proteins and RNA modifications includingprocessing, stability, splicing, and translational control.
We use the Octet® RED96.
Application protocols: easy to follow directions describing the step by step experimental procedure.
Antibody validation principles at Cell Signaling Technology.
In the CST video library, you can watch how-to videos from the Tech Tips, research webinars presented by CST scientists & relevant videos.
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy and light chain.
CIP-treated membranes are prepared with the following protocol:REAGENTS:Quick CIP - #M0525 (New England Biolabs)rCutSmart™ Buffer - #B6004 (New England Biolabs)Tris Buffered Saline with Tween® 20 (TBST-10X) #9997PROCEDURE:1) Solution Preparation. 1X TBST Wash Buffer: Prepare 1L of 1X TBST by adding 100ml of 10X TBST to 900ml of RODI water and mix well.2) A duplicate set of lysates are run on SDS-PAGE and transferred to nitrocellulose membrane.3) After the transfer of proteins to the nitrocellulose membranes and BEFORE THE BLOCKING STEP, prepare 2 incubation vessels:a) Control/No CIPb) Treated/+CIP4) To the control/No CIP vessel, add 900μl of RODI water and 100μl of the 10X rCutSmart™ Buffer that is supplied with the CIP for 1mL total. If more volume is required, these volumes can be scaled.5) To the treated/+CIP tube, add 850μl RODI water, 100μl 10X rCutSmart™ Buffer, and 50μl CIP for 1mL total. If more volu…
CUT&Tag delivers quality NGS data even if the signal from the Bioanalyzer or TapeStation System is low and the DNA library yield is low.
Links to sample preparation protocols: provided in pdf format (9-15 page documents).
Immunofluorescence-validated antibodies from CST Cell Signaling Technology
Meet the aspiring young scientists who won the 4th annual Rising Black Scientists Awards essay competition.
The privacy policy currently in place from Cell Signaling Technology, Inc.
The guarantee behind all of CST's antibody products, ensuring performance, lot-to-lot consistency, and application validation.
High-quality antibodies & assays supporting the interrogation of metabolic signaling pathways. Those required for energy, autophagy & biochemical reactions.
Antibodies and assays for the investigation of fibrosis coveringa variety of signaling pathways involved in extracellular matrixdeposition and immune responses.
General Manager of Cell Signaling Technology China, Jay Dong, is Elected Co-President of Chinese Society for Cell Biology’s Academia-Industry Consortium for Innovation and Entrepreneurship.
We have not back calculated the number of viable cells per well based on the OD readout from the XTT Cell Viability Kit #9095. We use the OD readout to see the activity level of the wells. We have been unable to find any calculations that could be of use for determining the number of viable cells from the OD readout. Unfortunately, we do not have any additional information to provide.
We at CST are excited to get our blog up and running. Our intent is to create a space where we can talk about what you should expect from your bench.
We catch up with last year's Rising Black Scientist Award recipients. If you'd like to be considered, submit your essay this week and tell your story!
How CST responds to customer feedback as it relates to development of the product portfolio.
Immunohistochemistry application information
Learn more about our approach to science and process for creating the best antibodies in the industry
CST diverts it through an innovative program that has enabled the company to recycle more than 4,400 pounds of gloves.
Navigating peer review can be challenging for early-career researchers. Here are tips for how to choose peer reviewers for your suggested reviewer list.
These five organizations received donations as part of our annual global giving program at CST, one of many ways we help the impoverished and underserved.
Cell Signaling Technology Chief Scientific Officer, Dr. Roby Polakiewicz, to Speak on Two Panels at the Global Biological Standards Institute (GBSI) Antibody Validation Workshop