Download a Post Translational Modification of Amino Acids Poster
Experienced CST scientists perform qualitative and quantitative profiling of post-translational modifications (PTMs) using proprietary proteomic methods that combine antibody enrichment of PTM-containing peptides with LC-MS/MS. The PTMScan® team works with you from project planning to delivery of a comprehensive data package that includes:
- Qualitative/quantitative tables
- Informatics tables
- Microsoft Powerpoint® summary
- Microsoft Word® guide to prioritizing follow-up candidates
TMT10plex™ Total Proteome profiling service provides accurate global profiling of protein abundance in cells and tissues, using multiplexed sample labeling with Tandem Mass Tags™ (TMT™) and liquid chromatography tandem mass spectrometry (LC-MS/MS).
PTMScan® Discovery is used to identify and quantify hundreds to thousands of PTM peptides.
IMAC captures negatively charged phosphopeptides using positively charged metal ions and is complementary to phosphorylation analysis by PTMScan® Discovery.
PTMScan® Direct is a mass-spectrometry-based antibody array used to quantitatively assay the activity of components of known signaling pathways across cell lines or treatments.
KinomeView® Profiling is a western blot prescreen that uses the same PTM and motif antibodies available for PTMScan Discovery.
Global Proteome Service
Quantification of protein levels across cell lines or treatments without enrichment for PTM-containing peptides.
Ingenuity® Pathway Analysis
Mapping of PTMScan results onto canonical pathways and new interaction networks using Qiagen’s Ingenuity® Pathway Analysis (IPA) software.
Contact us for more information about Proteomic Services
Estimated Completion Times for PTMScan® Projects
|Number of Samples||1 Motif Ab (Weeks)||2 Motif Abs (Weeks)||3 Motif Abs (Weeks)|
|greater than 42*||9–11||11–13||13–15|
Estimated Completion Times for KinomeView® Projects
|Number of Lanes||~20 Motif Ab + 3 Control Abs (Weeks)|
|greater than 42*||5–8|
* More accurate timelines for service projects with greater than 42 samples will be discussed during the consultation. NOTE: Estimated timelines are based on when the project is initiated by Cell Signaling Technology, not when the material is received from the client.