Alexa Fluor® 647 Antibody Conjugates
Multiplex more targets at once with the far-red fluorescence of Alexa Fluor 647 conjugates. Alexa Fluor 647, an ultra-bright fluorophore with high photostability and pH tolerance, limits background signal due to its excitation and emission wavelengths being outside the range where autofluorescence is common in biological samples.
Attributes
Excitation - 650 | Emission: 671 |
Laser Line: 594/633 nm | Common Filter Set: 660/20 |
Brightness: 5 | Quantum Yield: 0.33 |
The dashed red line shows the excitation spectrum for Alexa Fluor® 647, with the emission spectrum shown in solid red. For multiplexed labeling experiments, selecting antibodies conjugated to fluorophores with limited emission overlap yields the best results.
Applications in Research
Alexa Fluor 647 conjugates have several key advantages for researchers:
- The far-red emission spectrum of Alexa Fluor 647 enables imaging beyond the lipofuscin-based auto-fluorescent spectra which can be challenging when imaging in tissues and tumor samples.
- Alexa Fluor 647 superior brightness allows detection of low abundance antigens.
- Our Alexa Fluor 647 conjugates are ready-made for immediate application, limiting the burden on your time and resources.
- Available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.
The stability of Alexa Fluor 647 conjugates makes them applicable in a wide variety of experiments, including:
- Immunohistochemistry
- Immunofluorescence
- Flow cytometry and spectral flow cytometry
- Spatial biology
- FRET
- Time-Resolved FRET
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 647 conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our Alexa Fluor 647 Conjugated Antibodies
Looking for primary and secondary Alexa Fluor 647 antibody conjugates? Check out our wide selection of validated products or utilize our custom conjugation services to create the ideal solution for your needs.
Related or Alternative Fluorophores
Cy5 | APC | Dylight 650 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology, CST and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.