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Alexa Fluor® 700 Antibody Conjugates

Sharpen your immunofluorescence imaging with more signal and less background noise using Alexa Fluor 700 conjugates. The near-infrared emission spectrum of Alexa Fluor 700 makes it a powerful tool for multiplexed imaging and flow cytometry experiments due to its low spectral overlap with green and blue fluors. This highly photostable dye is compatible with standard 628-640 nm laser lines and delivers consistent and reliable results.

Attributes

Excitation: 696 nm

Emission: 716 nm

Laser Line: 628-640 nm

Common Filter Set: 730/45

Brightness: 2

Quantum Yield: 0.25

The absorption spectrum of Alexa Fluor 700 is represented by the dashed red line, and the emission spectrum is represented in solid red. For optimal results in multiplex labeling experiments, choose fluorophores with minimal overlap in excitation and/or emission spectra. Alexa Fluor 700 can be excited by standard 628-640 nm excitation lasers.

Applications in Research

There are multiple characteristics of Alexa Fluor 700 that help it excel in research:

  • The signal of Alexa Fluor 700 is dim, making it ideal for labeling abundant proteins in multiplexing experiments while using brighter fluorophores to mark less abundant molecular targets.
  • As a near-infrared fluorophore, Alexa Fluor 700's minimal autofluorescence and reduced light scattering provides greater flexibility for multiplexed panel design.
  • Available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 700 has compatibility with several detection systems and is a good choice for:

Generate high contrast, low background images

Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 700 Conjugate) #64522 (red), DyLight 488 Phalloidin #12935 (green), and DAPI #4083 (blue). Prior to immunostaining, antibody was challenged with no peptide (left), or peptide corresponding to phosphorylated Histone H3 (Ser10) (right).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 700 conjugates. CST uses a stringent and reproducible manufacturing process that results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.


Explore Our Alexa Fluor 700 Conjugated Antibodies

Looking for Alexa Fluor 700-conjugated primary and secondary antibodies? Browse our wide selection of validated products, or use our custom conjugation services to create the ideal solution for your needs.

Related or Alternative Fluorophores

iFluor® 700

CF 700

Brilliant Violet 711

For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology, CST and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.