Alexa Fluor® 750 Antibody Conjugates
Achieve superior tissue penetration in your imaging workflows with Alexa Fluor 750 antibody conjugates. This highly photostable fluor is ideal for in vivo imaging due to its low autofluorescence in biological samples. The NIR emission effectively separates from other red-emitting dyes while limiting background noise. Alexa Fluor 750 is also pH insensitive over a wide molar range, making it suitable for various experimental setups.
Attributes
Excitation: 752 nm | Emission: 776 nm |
Laser Line: 633, 730-750 nm | Common Filter Set: 780/60 |
Brightness: 4 | Quantum Yield: 0.12 |
The excitation spectrum (dashed red line) and emission spectrum (solid red) of Alexa Fluor 750 are shown above. Its high photostability and reduced autofluorescence ensure consistent and reliable signals during prolonged imaging sessions. Selecting antibodies conjugated to fluorophores with minimal excitation or emission overlap is crucial for optimal results in multiplex labeling experiments.
Applications in Research
Several aspects of Alexa Fluor 750 make it a valuable research tool:
- Alexa Fluor 750 can be differentiated from other NIR signals, making it possible to do even more extensive multiplexed flow cytometry experiments.
- Excels in live imaging with its robust NIR-signal that reduces light scattering and penetrates tissue for accurate cellular and molecular analysis, particularly in dense and intricate tissue samples.
- Available with various reactive groups to allow conjugation with most biomolecules; permitting access to amine, thiol, glycan, or other conjugation chemistries.
Alexa Fluor 750 is insensitive to changes in pH over a broad molar range and is suitable for multiplexed applications across several detection systems:
- Immunohistochemistry
- Flow cytometry and spectral flow cytometry
- Immunofluorescence
- Spatial biology
- FRET
- Western blotting
Achieve peak performance in flow cytometry
Flow cytometric analysis of fixed/permeabilized HeLa cells using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Alexa Fluor® 750 Conjugate) #98316 (solid line) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 750 Conjugate) #90999 (dashed line).
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 750 conjugates. CST uses a stringent and reproducible manufacturing process that results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our Alexa Fluor 750 Conjugated Antibodies
Take advantage of our custom conjugation services to build the perfect Alexa Fluor 750 primary or secondary antibody, or explore our extensive catalog of ready-made, validated products.
Related or Alternative Fluorophores
Cy®7 | APC-Fire 750 | CF 750 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. Cy is a registered trademark of GE Healthcare. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.