PerCP Antibody Conjugates

Choose PerCP conjugated antibodies to optimize your flow cytometry experiments. With a maximum excitation wavelength of 480 nm, it can be coupled with common fluors like FITC and Alexa Fluor^® 488 using the 488 nm laser line without emission overlap. While generally a more cost-effective option, PerCP is sensitive to photobleaching, limiting its use in experiments requiring long-term imaging.

Flow cytometric analysis of live human peripheral blood mononuclear cells using CD19 (SJ25C1) Mouse mAb (PerCP Conjugate) #17634 (right) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PerCP Conjugate) #97713 (left), co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881.

Attributes

The separation of excitation (dashed red line) and emission (solid red) maxima can be leveraged for multiplexed labeling experiments using a single laser line. For multiplexed labeling experiments, selecting antibodies conjugated to fluorophores with limited excitation and/or emission overlap yields the best results.

Applications in Research

While not as versatile as other red-emitting fluors, PerCP has many characteristics that make it a valuable tool for your research, including:

• Emission in the red region (675 nm) reduces spectral overlap with common visible-light fluorophores.

• Excited by a standard 488 nm laser, commonly found in most flow cytometers and fluorescence microscopy setups.

• Optimal for surface-level fluorescence detection and cell suspension assays where its lower brightness and photobleaching sensitivity are less of a concern because of the short duration of laser exposure.

Based on its characteristics, PerCP conjugates can be readily deployed for the following experiments:

• Flow cytometry and spectral flow cytometry

• Western blotting

Simplify your flow cytometry workflows with PerCP

Flow cytometric analysis of live human peripheral blood mononuclear cells using HLA-DR (L243) Mouse mAb (PerCP Conjugate) #17634 (solid line) compared to concentration-matched Mouse Isotype Control (PerCP Conjugate) (dashed line).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use PerCP antibody conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our PerCP Conjugated Antibodies

Do you need ready-made PerCP antibody conjugates? If you don’t find what you need, take advantage of our custom conjugation services to develop the perfect antibody conjugate for your research.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology and CST are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Coporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}