NZF Protein Domain

The NZF (Npl4 Zinc Finger) domain is a compact zinc-binding module found in many proteins involved in ubiquitin-dependent processes. The approximately 35 amino acid NZF domain forms a compact module composed of four anti-parallel β-strands linked by three ordered loops organized about a rubredoxin-like Zn(Cys)₄ metal-binding site. NZF domains typically contain an x(4)-W-x-C-x(2)-C-x(3)-N-x(6)-C-x(2)-C-x(5) consensus sequence. The NZF domain is found in more than 100 proteins, while numerous other proteins containing recognizable variants of the NZF motif. While many NZF domains bind mono- and polyubiquitin, not all NZF domains can bind ubiquitin.