For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Products available from Cell Signaling Technology are linked by their respective catalog numbers.
NOTE: Prepare solutions with Milli-Q or equivalently purified water.
A general protocol for sample preparation is described below.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
Skip to Detection of Proteins (Step D).
posted June 2005
revised October 2008